Functional Properties of the Apical Na+-K+-2Cl− Cotransporter Isoforms
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چکیده
منابع مشابه
Transport and regulatory properties of the apical Na-K-2Cl cotransporter of macula densa cells.
[Formula: see text]/NH3fluxes were used to probe apical Na-K-2Cl transport activity of macula densa (MD) cells from rabbit kidney. In the presence of 25 mM NaCl and 5 mM Ba2+, addition of 20 mM[Formula: see text] to the lumen produced a profound intracellular acidification, and ∼80% of the initial acidification rate was bumetanide sensitive. The[Formula: see text]-induced acidification rate was...
متن کاملIsoforms of the Na-K-2Cl cotransporter in murine TAL I. Molecular characterization and intrarenal localization.
We have identified several alternatively spliced cDNAs encoding mBSC1, an apical bumetanide-sensitive Na+-K+-2Cl-cotransporter from mouse kidney. Two full-length clones were isolated, designated C4 and C9, predicting proteins of 770 and 1,095 amino acids, respectively. The C4 isoforms are generated by utilization of an alternative polyadenylation site located within the intron between exons 16 ...
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We studied the phosphorylation of the secretory Na(+)-K(+)-2Cl(-) cotransporter (NKCC1) in rat parotid acinar cells. We have previously shown that NKCC1 activity in these cells is dramatically upregulated in response to beta-adrenergic stimulation and that this upregulation correlates with NKCC1 phosphorylation, possibly due to protein kinase A (PKA). We show here that when ATP is added to puri...
متن کاملIsoforms of the Na-K-2Cl cotransporter in murine TAL II. Functional characterization and activation by cAMP.
The functional properties of alternatively spliced isoforms of the mouse apical Na+-K+-2Cl-cotransporter (mBSC1) were examined, using expression in Xenopus oocytes and measurement of22Na+or86Rb+uptake. A total of six isoforms, generated by the combinatorial association of three 5' exon cassettes (A, B, and F) with two alternative 3' ends, are expressed in mouse thick ascending limb (TAL) [see c...
متن کاملAnnexin A2 mediates apical trafficking of renal Na⁺-K⁺-2Cl⁻ cotransporter.
The furosemide-sensitive Na(+)-K(+)-2Cl(-) cotransporter (NKCC2) is responsible for urine concentration and helps maintain systemic salt homeostasis. Its activity depends on trafficking to, and insertion into, the apical membrane, as well as on phosphorylation of conserved N-terminal serine and threonine residues. Vasopressin (AVP) signaling via PKA and other kinases activates NKCC2. Associatio...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2002
ISSN: 0021-9258
DOI: 10.1074/jbc.m110442200